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Title: Wet-electrospun PCL/PLLA Blend Scaffolds: Effects of Versatile Coagulation Baths on Physicochemical and Biological Properties of the Scaffolds
Journal: Regeneration, Reconstruction & Restoration
Author: 1. Majid Salehi, 2. Mahdi Naseri-Nosar, Mahmoud Azami, 3. Sadegh Ghorbani, 4. Saeed Farzamfar
Year: 2017
Address: 1. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud 3613773955, Iran 2. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran 1417755469, Iran 3. Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares University, Tehran 14115111, Iran 4. Department of Medical nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran 1417755469, Iran
Abstract: High surface/volume ratio and 3-dimensionality of nanofibers increases cell-scaffold interactions and promote migration and proliferation of cells. Wet electrospinning is a variant of electrospinning technology that is utilized to produce nanofibrous scaffolds. Altering the parameters governing the wet electrospinning process such as applied voltage, polymer concentration, composition and depth of the coagulation bath, and tip to bath distance can affect the morphology of the produced scaffolds. In this study, the influence of various coagulation baths on the physicochemical properties of the wet-electrospun nanofibers was investigated. Materials and Methods: Poly (ε-caprolactone)/Poly (L-lactic) acid 15% (w/v) blends under an applied voltage of 15 kV, and a tip-to-bath distance of 10 cm. were used to prepare fibrous scaffolds via wetelectrospinning technique into aqueous solution of sodium hydroxide (NaOH) (pH~13), distilled water, ethanol, water/ethanol (3:7) (v/v) and water/ethanol/methanol (6:2:2) (v/v). The final products were characterized by scanning electron microscopy (SEM), liquid displacement technique, contact angle measurement, compressive and tensile tests. As well as, cell adhesion and cell viability through human adipose-derived stem cells (hADSCs) cell culture. Results: Wet-electrospun fibers, except in the almost fully beaded structure of water/ethanol (3:7) (v/v) specimen exhibited random, dispersive and non-woven morphology under SEM observation. The coagulation bath composition significantly influenced on porosity, wettability, mechanical properties and biocompatibility of the scaffolds. The porosity measurement via liquid displacement method showed that except for the specimen in which the blend was spun into NaOH, other scaffolds could not meet the accepted ideal porosity percentage of above 80%. According to the contact angle measurement data, it was expected that all scaffolds experience low cellular attachment and proliferation. Conversely, in vitro hADSCs culture demonstrated that the scaffolds presented a non-toxic environment and enhanced cell proliferation and attachment. Conclusion: The data indicated that the scaffold spun into NaOH was the best candidate among other specimens to culture hADSCs.
Keywords: Human adipose-derived stem cells; Poly (ɛ-caprolactone)/Poly (L-lactic) acid; Scaffold; Wet-electrospinning
Application: Scaffold, Tissue Engineering
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