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Title: Sciatic nerve regeneration by transplantation of Schwann cells via erythropoietin controlled‚Äźreleasing polylactic acid/multiwalled carbon nanotubes/gelatin nanofibrils neural guidance conduit
Journal: Journal of Biomedical Materials Research Part B: Applied Biomaterials
Author: 1. Majid Salehi, Mahdi Naseri-Nosar, Somayeh Ebrahimi-Barough, Jafar Ai, 2. Mohammdreza Nourani, 3. Arash Khojasteh, 4. Amir-Ali Hamidieh, 5. Amir Amani, Saeed Farzamfar
Year: 2017
Address: 1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran 1417755469, Iran 2. Nano Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran 1435944711, Iran 3. Dental Research Centre, Research Institute of Dental Sciences, School of Dentistry, Shaheed Beheshti University of Medical Sciences, Tehran 1983969411, Iran 4. Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran 1411713135, Iran 5. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran 1417755469, Iran
Abstract: The current study aimed to enhance the efficacy of peripheral nerve regeneration using an electrically conductive biodegradable porous neural guidance conduit for transplantation of allogeneic Schwann cells (SCs). The conduit was produced from polylactic acid (PLA), multiwalled carbon nanotubes (MWCNTs), and gelatin nanofibrils (GNFs) coated with the recombinant human erythropoietin-loaded chitosan nanoparticles (rhEpo-CNPs). The PLA/MWCNTs/GNFs/rhEpo- CNPs conduit had the porosity of 85.7860.70%, the contact angle of 77.6561.918 and the ultimate tensile strength and compressive modulus of 5.5160.13 MPa and 2.6660.34 MPa, respectively. The conduit showed the electrical conductivity of 0.32 S cm21 and lost about 11% of its weight after 60 days in normal saline. The produced conduit was able to release the rhEpo for at least 2 weeks and exhibited favorable cytocompatibility towards SCs. For functional analysis, the conduit was seeded with 1.5 3 104 SCs and implanted into a 10 mm sciatic nerve defect of Wistar rat. After 14 weeks, the results of sciatic functional index, hot plate latency, compound muscle action potential amplitude, weight-loss percentage of wet gastrocnemius muscle and Histopathological examination using hematoxylin-eosin and Luxol fast blue staining demonstrated that the produced conduit had comparable nerve regeneration to the autograft, as the gold standard to bridge the nerve gaps.
Keywords: chitosan nanoparticles, erythropoietin, neural guidance conduit, polylactic acid, multi-walled carbon nanotubes, Schwann cells
Application: Tissue Engineering
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URL:"" & "/doi/10.1002/jbm.b.33952/full"#