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Title: Comparison of Capability of Human Bone Marrow Mesenchymal Stem Cells and Endometrial Stem Cells to Differentiate into Motor Neurons on Electrospun Poly (Ε-Caprolactone) Scaffold
Journal: Molecular neurobiology
Author: 1. Hooshang Saberi, Abbas Norouzi-Javidan, Babak Arjmand, 1,2,3. Sadegh Shirian, 1,4. Jafar Ai, 3. Sayed Mostafa Modarres Mousavi, 4. Somayeh Ebrahimi-Barough, 5. Mohammad Ali Derakhshan.
Year: 2015
Address: 1. Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran. 2. Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran. 3. Shefa Neuroscience Research Center, Khatam-Al-Anbia Hospital, Tehran, Iran. 4. Department of Tissue Engineering, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. 5. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Abstract: Human endometrial and bone marrow-derived mesenchymal stem cells can be differentiated into a number of cell lineages. Mesenchymal stem cells (MSCs) are potential candidates for cellular therapy. The differentiation of human bone marrow MSCs (hBM-MSCs) and endometrial stem cells (hEnSCs) into motor neuron-like cells has been rarely investigated previously; however, the comparison between these stem cells when they are differentiated into motor neuronlike cell is yet to be studied. The aim of this study was therefore to investigate and compare the capability of hBM-MSCs and hEnSCs cultured on tissue culture polystyrene (TCP) and poly ε-caprolactone (PCL) nanofibrous scaffold to differentiate into motor neuron-like cells in the presence of neural inductive molecules. Engineered hBM-MSCs and hEnSCs seeded on PCL nanofibrous scaffold were differentiated into beta-tubulin III, islet-1, Neurofilament-H (NF-H), HB9, Pax6, and choactase-positive motor neurons by immunostaining and real-time PCR, in response to the signaling molecules. The data obtained from PCR and immunostaining showed that the expression of motor neuron markers of both hBM-MSCs and hEnSCs differentiated cells on PCL scaffold are significantly higher than that of the control group. The expression of these markers in hEnSCs differentiated cells was higher than that in hBM-MSCs. However, this difference was not statistically significant. In conclusion, differentiated hBM-MSCs and hEnSCs on PCL can provide a suitable threedimensional situation for neuronal survival and outgrowth for regeneration of the central nervous system. Both cells may be potential candidates for cellular therapy in motor neuron disorders. However, differentiation of hEnSCs into motor neuron-like cells was better than hBM-MSCs.
Keywords: Bone marrow mesenchymal stem cell . Endometrial stem cell . Motor neuron-like cell . Signaling molecules . PCL nanofibrous scaffold . Differentiation
Application: Scaffold
Product Model 1: Electroris
Product Model 2:
URL: https://link.springer.com/article/10.1007/s12035-015-9442-5#="https://link.springer.com" & "/article/10.1007/s12035-015-9442-5"#