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Title: Biosynthesis and physicochemical characterization of a bacterial polysaccharide/polyamide blend, applied for microfluidics study in porous media
Journal: International journal of biological macromolecules
Author: 1. Maryam Ijadi Bajestani, Seyyed Mohammad Mousavi, Seyed Abbas Shojaosadati 2. Arezou Jafari
Year: 2017
Address: 1. Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, Tehran, Iran 2. Petroleum Engineering Group, Chemical Engineering Department, Tarbiat Modares University, Tehran, Iran
Abstract: Screening among some new isolated bacteria from oily samples, which were capable of producing extracellular polymeric substances (EPSs), one was selected and identified as Bacillus sonorensis. An efficient micro-total analysis approach was carried out to assay the produced EPSs by this bacterium. Sucrose and yeast concentrations as carbon and nitrogen sources, respectively, sodium salt concentration and initial pH were selected to be the variables in experimental design. Production of EPS in optimal condition was increased by 5.3 times. Further EPS purification was carried out to identify the biopolymers. The bacteria produced high molecular weight biopolymers with a number average molecular weight (M̅ n) of 9.1×106 g/mole determined by gel permeation chromatography (GPC). Biopolymer characterization demonstrated the biosynthesis of both polysaccharides and polyamides by the bacteria. For the biopolymer blend, thermal properties and morphological characteristics were studied using simultaneous differential scanning calorimetric and thermal gravimetric analyses (DSC/TGA) and field emission scanning electron microscope (FESEM) analyses. Finally, the biopolymer blend was injected into an oil saturated glass micro model to study the enhancement of oil recovery by biopolymer flooding in contrast with water flooding. It was found that oil recovery increased by 36%, from 23% using water flooding to 59% for biopolymer injection.
Keywords: Bacillus sonorensis, Polysaccharide/Polyamide blend; Physicochemical properties; Enhanced oil recovery
Application: Extraction
Product Model 1: Syringe Pump
Product Model 2:
URL: http://www.sciencedirect.com/science/article/pii/S0141813016311722#="http://www.sciencedirect.com" & "/science/article/pii/S0141813016311722"#