ISI Papers With Our Products
Title: Cellular Response of Limbal Stem Cells on Polycaprolactone Nanofibrous Scaffolds for Ocular Epithelial Regeneration
Journal: Current eye research
Author: 1. Alireza Baradaran-Rafii, 2. Esmaeil Biazar, 3. Saeed Heidari-keshel
Year: 2016
Address: 1. Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2. Department of
Biomaterials Engineering, Islamic Azad University, Tonekabon Branch, Iran
3. Proteomics Research Center,
Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract: Purpose: The aim of this study was to develop nanofibrous polycaprolactone (PCL) substrate for limbal stem cell
(LSC) expansion that can serve as a potential alternative substrate to replace human amniotic membrane (AM).
Materials and methods: The human limbus stem cell was used to evaluate the biocompatibility of substrates
(nanofibrous scaffold and, human AM) based on their phenotypic profile, viability, proliferation and
attachment ability.
Results: Biocompatibility results indicated that the all substrates were highly biocompatible, as LSCs could
favorably attach and proliferate on the nanofibrous surface. Microscopic figures showed that the human LSCs
were firmly anchored to the substrates and were able to retain a normal corneal stem cell phenotype.
Microscopic analyses illustrated that cells infiltrated the nanofibers and successfully formed a threedimensional
corneal epithelium, which was viable for two weeks. Immunocytochemistry (ICC) and real
time-PCR results revealed no change in the expression profile of LECs grown on nanofibrous substrate when
compared to those grown on human AM.
Conclusion: In addition, electrospun nanofibrous PCL substrate provides not only a milieu supporting LSCs
expansion, but also serve as a useful alternative carrier for ocular surface tissue engineering and could be used
as an alternative substrate to AM.
Keywords: Cellular analyses, cornea regeneration, limbal stem cells, nanofibrous scaffold, polycaprolactone
Application: Scaffold
Product Model 1: Electroris
Product Model 2:
URL: http://www.tandfonline.com/doi/abs/10.3109/02713683.2015.1019004?journalCode=icey20#="http://www.tandfonline.com" & "/doi/abs/10.3109/02713683.2015.1019004?journalCode=icey20"#